Year : 2020 Volume : 35 Issue : 3

Full Text (PDF)

Antibacterial Activity of Cyanobacteria Dolichospermum affine Isolated from Freshwater

Open Access

Abstract

Cyanobacteria are known for their potential for antibacterial activity against a variety of pathogens, which are of medicinal importance in drug development. In addition, Cyanobacterial species produce various secondary metabolites that are used as dye and pigmentation and food additives. Cyanobacteria Dolichospermum affine (Lemmermann) Wacklin, L. Hoffmann & Komárek was isolated from freshwater resources and its antimicrobial effect was studied. Chloroform, methanol and water extracts of D. affine were tested to investigate their efficiency against five pathogenic bacterial strains [Pseudomonas aeruginosa (ATCC 27853), Shigella dysenteriae (ATCC 11835), Escherichia coli (ATCC 25924), Staphylococcus aureus (ATCC 29213) and Bacillus subtilis (ATCC 6633)]. The antimicrobial test was determined using the disk diffusion method. The antimicrobial activities of D. affine extracts were measured using the diameter of the inhibition zone (DIZ) of the pathogen microorganisms. The results showed that B. subtilis and E.coli were more sensitive, while S. aureus and P. aeruginosa showed more intermediate results. The highest antimicrobial activity was measured against E. coli (DIZ=13.9±0.05 mm - methanol), followed by B. subtilis (DIZ=13.6±0.05 mm - methanol). The lowest antibacterial effect of D. affine extracts were observed against P. aeruginosa (DIZ=11.7±0.02 mm - chloroform) and S. aureus (DIZ=12.2±0.03 mm - chloroform). The Gram-negative bacteria S. dysenteria exhibited no zone of inhibition. The aqueous extract showed poor activities against the tested pathogenic bacteria. Therefore, this study revealed that D. affine extracts would be a promising natural resource for new antibiotics and further research would be needed.

Keywords

Cyanobacteria   Dolichospermum-affine   antibacterial-activity   zone-of-inhibition  

Corresponding Author

Dilek Yalçın

References

  • Abd El-Aty, A. M., Mohamed, A. A. & Samhan, F. A. (2014). In vitro antioxidant and antibacterial activities of two fresh water Cyanobacterial species, Oscillatoria agardhii and Anabaena sphaerica. Journal of Applied Pharmaceutical Science, 4(7), 69-75.
  • Abobaker, H. M. & Elsalhin, H. E. L. (2019). Antibacterial Activity of Anabaena circinalis Isolated from Fresh Water. Journal of Advances in Microbiology, 15(3), 1-7. [CrossRef]
  • Andersen, R. A. & Kawachi, M. (2005). Traditional microalgae isolation techniques. In R. A. Andersen (Ed), Algal culturing techniques (pp. 83-100). London, Elsevier Press. [CrossRef]
  • Brennan, L. & Owened, P. (2010). Biofuels from microalgae A review of technologies for production, processing and extractions of biofuels and co-products. Renewable and Sustainable Energy Reviews, 14(2), 557-577. [CrossRef]
  • Converti, A., Casazza, A. A., Ortiz, E. Y., Perego, P. & Borghi, M. D. (2009). Effect of temperature and nitrogen concentr
Daha Fazla Göster

Details

DOI 10.26650/ASE2020646338

Submission : 19 Kas 2019

Early Viewed : 25 May 2020

Full Text (PDF)